Shang-Hsun Yang
Department of Physiology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
Title: A miracle (miR-196a) in the fight against Huntington Disease
Biography
Biography: Shang-Hsun Yang
Abstract
Huntington’s disease (HD) is a genetic disease, and caused by a mutation in Huntingtin gene, leading to neuropathological symptoms. To date, there is no effective medicine for HD. Based on previous studies, transcriptional regulation is impaired during the progression of HD, and regulation of microRNA(miRNA) is one of affected mechanisms. Since HD leads to dysfunction of gene regulation and one miRNA could target to multiple pathways, it suggests miRNA could be one potential treatment for HD. In my laboratory, we identified one potential miRNA, miR-196a, from HD transgenic monkeys, and found the neuroprotective effects of miR-196a on HD in cell, transgenic mouse and HD patient-derived induced pluripotent stem cell models. miR-196a could not only improve molecular, neuropathological and behavioral phenotypes in transgenic mouse models, but also suppress pathological aggregates in neurons derived from HD patients. Furthermore, we also investigate molecular mechanisms of miR-196a, and show miR-196a could enhance cellular morphology, intracellular transport, synaptic plasticity, neuronal activity, learning and memory both in vitro and in vivo. In addition, miR-196a could work through binding to 3’ untranslated region of RAN binding protein 10 (RANBP10) to suppress the protein expression, further enhancing the assembly of β-tubulin. Most importantly, overexpression of RANBP10 led to worse neuronal morphology and severer pathological phenotypes in the HD transgenic mouse model, suggesting that miR-196a enhances neuronal morphology through suppressing RANBP10 to provide neuroprotection in HD. These results suggest the important role of miR-196a on HD, and might provide a new insight of therapeutical strategy for HD